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Research

Flow Cytometry Core Facility

The HSS Research Institute provides flow cytometric analysis services in its location at 515 East 71st street, and both cell sorting and analysis in the Core Laboratories Center (CLC) Flow Cytometry Core Facility located in the Weill Cornell Medicine building located at 1300 York Avenue.

HSS Research Institute FACS Canto

The HSS Research Institute is equipped with a FACS Canto for analysis by HSS users only. 
Outside users should contact Dr. Risë Schwab with questions, SchwabR@HSS.edu

Location and Contact Information

The core director is Dr. Michela Manni
Email: ManniM@HSS.edu

HSS Research Institute
515 East 71st Street
Room 224

Scheduling Instrument Time

First time users must contact Dr. Manni for training before scheudling access will be granted.

To reach the sign-up calendar for this instrument, please click on the “SIGN UP” link at the end of this section. Alternatively, e-mail ManniM@HSS.edu to reserve time.

Please allow sufficient time for instrument start-up (about ten minutes) and post-experiment instrument cleaning and data backup (usually about 20 minutes) when calculating your estimated usage. Please sign up only for the time you really need; all users will be billed for any time signed up for, regardless of whether the time was actually used. If you need to cancel or modify a scheduled run, please follow the instructions on the “SIGN UP” link.

Carefully follow the posted basic preventive maintenance instructions. When you have completed your experiment, please remember to flush the instrument with 10% bleach and distilled water according to the posted directions. Each user should empty the waste tank and refill the sheath after use. Sheath fluid is provided by the Core. These directions must be followed, no matter how small your experiment. Following these simple maintenance instructions will greatly enhance day-to-day instrument performance and minimize downtime for servicing. Repeated failure to take care of the instrument in this manner will necessitate frequent replacement of the fluidic system, with resultant instrument downtime and increased user costs.

Monday mornings from 10:00 am to 12 noon are reserved by the Core Director for routine preventive maintenance. Please do not plan to use the instruments during this time.

CLC Flow Cytometry Core Facility

Continuing the long relationship between HSS and Weill Cornell Medicine (WCM) in providing a shared flow cytometry core facility, core instruments and service have been expanded into a new Core Laboratories Center (CLC) Flow Cytometry Core Facility. The core has available two sorters (FACSAria and Influx) and two analytical cytometers (FACSCalibur and FACScan).  All instruments are available to investigators through the core’s instrument scheduling tools on the iLab portal (https://wcmc.corefacilities.org).  First time users should contact the core for training before signing up to use these instruments.

The CLC Flow Cytometry Core Facility offers the following instruments and capabilities:

  • BD Influx*: (1) a fully enclosed BSL-2 rated hood; (2) 355, 405, 445, 488, 561, and 640 laser lines; (3) 22 fluorescence detectors; (4) sorts cell sizes 1-50 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 6-way simultaneous sorting (tubes only); and (7) customizable fluidics to accommodate sensitive cells.
  • BD FACSAria II SORP: (1) a fully enclosed BSL-2 rated hood; (2) 355, 405, 488, 561, and 640 laser lines; (3) 18 fluorescence detectors; (4) sorts cell sizes 1-35 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 4-way simultaneous sorting; and (7) unassisted operation for trained core users.
  • BD/Cytek FACSCalibur DxP 11: (1) 405, 488, 561, and 640 laser lines; (2) 11 fluorescence detectors; (3) analysis from single tubes, tube carousel, and 96-well plates; (4) unassisted operation for trained core users.
  • BD FACScan: (1) 488 and 640 laser lines; (2) 4 fluorescence detectors; (3) analysis from single tubes; (4) unassisted operation for trained core users.

The core is located on the 6th floor of the WCM “A” building and the sub-basement level of the “E” building at 1300 York Avenue.

*Be sure to include the following citation in publications that use data generated using the BD Influx:
"This work includes data generated using a BD Influx Cell Sorter that was purchased with funding from the National Institutes of Health SIG grant S10OD019986 award to Hospital for Special Surgery"
Please remember to link the citation with the S10 award in My NCBI

For more information about these instruments and services available go to:
http://corefacilities.weill.cornell.edu/flow_cyto.html

To arrange for training and for assistance, contact the CLC Flow Core Managers: Jason McCormick at jam2073@med.cornell.edu or Tom Baumgartner at tob2008@med.cornell.edu.