Enhancement of Osseointegration Using a Mouse Model


Mathias P. Bostrom, MD
Xu Yang, MD

Project Description

According to the Agency for Healthcare Research and Quality, more than 285,000 total hip and 600,000 knee replacements are performed each year in the United States.  In the 1980s, cementless implants were introduced. Direct bone ingrowth and ongrowth, namely osseointegration, to the implant led to a more durable implant-bone interface than possible with cement and avoided the complications caused by cement insertion and the troublesome removal of cement during revision surgery.  Because cementless implants depend on new bone growth for stability, they require a longer healing time than cemented replacements. Many studies have been performed to optimize implant geometry, and surface topography, texture and coating to maximize new bone growth. However, the longevity of these implants is not always predictable. One cause of failure is the implant instability caused by suboptimal osseointegration. Our lab has developed a mouse model using a titanium porous implant inserted to proximal tibia to mimic the knee arthroplasty. With this model, we will investigate factors and cell-signaling pathways which affect and regulate osseointegration. We will also test the efficacy of various anabolic and anti-resorptive agents on osseointegration. Histomorphometry, back scattered electron microscopy, microCT, mechanical testing will be used to asses bone quantity and quality. Immunohistochemistry and rtPCR will be used to detect signaling pathways.The summer student will assist with mouse surgery, administration of agents to mice, tissue collection, and data analysis.

This position has been filled.