The FTIR Imaging Core has a Spectrum Spotlight FTIR imaging system. This technology utilizes PerkinElmer’s patented Dual Mode Detector (DMD). DMD provides significant performance advances compared to Infrared array detectors (64x64) previously used in this core. The Spotlight is capable of fast continuous collecting variable image size with spatial resolution of 6.25 and 25um and single-point IR microscopy. Wavelengths down to 720cm-1 can be imaged, allowing analyses of carbonate substitutions for phosphate in mineralized specimens. A full suite of powerful software tools is available. No probe molecule is required. It is non-destructive, thus results may be readily compared and contrasted against histology and histomorphometric data obtained on the very same section.
Human Iliac Crest Biopsy
Mineral spatial distribution
Infrared imaging can provide quantitative and qualitative information on the amount and nature of mineral and matrix present (corresponding to ash-weight and porosity measurements), mineral crystallinity (crystallite size), acid phosphate and carbonate substitute, relative amount of collagen crosslinks, orientation of collagen fibers, collagen integrity, collagen glycosylation, proteoglycans, lipids, protein secondary structure, and DNA/RNA. We have applied this technique to a variety of normal and diseased tissues including: bone, dentin, enamel, cartilage, meniscus, tendon, skin, blood vessels, and osteoblast and chondrocyte cell cultures. Materials such as polyethylene, implant devices and graft materials (osteoinduction vs osteoconduction; ossification vs calcification, comparing and contrasting tissue generated by the graft material to the host tissue), and tissue-engineered constructs have also been evaluated.
Collagen spatial distribution
Infrared imaging may be applied to the evaluation of human and animal tissues in a variety of diseases such as osteoporosis, osteoarthritis, osteogenesis imperfecta, osteopetrosis, atherosclerosis, and diabetes, and to the evaluation of various therapeutic protocols for these diseases. It can also be utilized in studies of protein adsorption and cellular/bacterial anchoring onto surfaces (biomineralization mechanisms, osteoinduction, pellicle and calculus formation, contact lenses), as well as dental tissues (dental caries, dentin, dentin bonding agents).
Infrared Images of Articular Cartilage
NIH Funded Users
Data Analysis by Core: $65/hour
Non-NIH Funded Academic Users
Data Analysis by Core: $75/hour
Computer usage for Data Analysis by Core User is free of charge.
Instrument and Data Analysis Training: $100/hour
Note: Publications that include data generated using the services of the Imaging Core must acknowledge support through NIH AR046121.
Adele Boskey, PhD
Mila Spevak, IR Imaging Core Technician