Mineralized Tissue Laboratory

Musculoskeletal Integrity Program

Mineralized Tissue Laboratory

The Mineralized Tissues Laboratory conducts studies on the mechanisms of biologic calcification in health and disease and on the changes that occur in mineralized tissues during development. Research methodologies include cell culture, x-ray diffraction, FTIR spectroscopy, FTIR micro-spectroscopy and imaging, micro-computed tomography, TEM and SEM, along with techniques of cell and molecular biology, image analysis, and solution chemistry.

Current investigations are focusing on the roles of collagen and noncollagenous extracellular matrix proteins in the regulation of bone and tooth mineralization based on solution studies and studies in transgenic animals; the effects of cell and matrix age and composition on in vitro mineral initiation in a model of endochondral ossification, and the changes in the composition of mineral and matrix in osteoporotic and normal bones before and after treatment with anti-resorptive and anabolic agents.

Selected Publications

Burket J, Gourion-Arsiquaud S, Havill LM, Baker SP, Boskey AL, van der Meulen MC. Microstructure and nanomechanical properties in osteons relate to tissue and animal age. J Biomech. 2010 Nov 11. [Epub ahead of print]
Reumann MK, Nair T, Strachna O, Boskey AL, Mayer-Kuckuk P. Production of VEGF receptor 1 and 2 mRNA and protein during endochondral bone repair is differential and healing phase specific. J Appl Physiol. 2010 Oct 14. [Epub ahead of print]
Boskey AL, Coleman R. Aging and Bone. J Dent Res. 2010 Oct 5. [Epub ahead of print]
Donnelly E, Chen DX, Boskey AL, Baker SP, van der Meulen MC. Contribution of mineral to bone structural behavior and tissue mechanical properties. Calcif Tissue Int. 2010 Nov;87(5):450-60.
Roy R, Kudryashov V, Binderman I, Boskey AL. The role of apoptosis in mineralizing murine versus avian micromass culture systems. J Cell Biochem. 2010 Oct 15;111(3):653-8.
Hosogane N, Huang Z, Rawlins BA, Liu X, Boachie-Adjei O, Boskey AL, Zhu W. Stromal derived factor-1 regulates bone morphogenetic protein 2-induced osteogenic differentiation of primary mesenchymal stem cells. Int J Biochem Cell Biol. 2010 Jul;42(7):1132-41.
Gericke A, Qin C, Sun Y, Redfern R, Redfern D, Fujimoto Y, Taleb H, Butler WT, Boskey AL. Different forms of DMP1 play distinct roles in mineralization. J Dent Res. 2010 Apr;89(4):355-9.
Gourion-Arsiquaud S, Faibish D, Myers E, Spevak L, Compston J, Hodsman A,Shane E, Recker RR, Boskey ER, Boskey AL. Use of FTIR spectroscopic imaging to identify parameters associated with fragility fracture. J Bone Miner Res. 2009 Sep;24(9):1565-71

Laboratory Personnel
Faculty
Mathias Bostrom, MD, Senior Member
Stephen B Doty, PhD, Senior Member
Daniel E. MacDonald, DDS, Adjunct Associate Member
Philipp Mayer-Kuckuk, Assistant Member
Cathleen L Raggio, MD, Affiliated Clinician
Bruce Rapuano, PhD, Assistant Member
Wei Zhu, PhD, Assistant Member

Postdoctoral Fellows: Rhima Coleman, PhD
Eve Donnelly, PhD
Ignacio Garcia, PhD
Kostas Verdelis, DDS
Research Associates Lyudmila Spevak Lyudmila Lukashova
Graduate Students: Ericka Calton

Director
Adele Boskey, PhD